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Biomedical engineering is a broad and interdisciplinary field that prepares graduates for a variety of careers across multiple career sectors. Given this breadth, undergraduate degree programs often have formal or informal opportunities for students to further specialize within the biomedical engineering major to develop skills in subdisciplines of biomedical engineering. While previous work has explored factors that influence student decision-making of engineering major choice, including the role of gender, limited work has explored factors that influence intra-major specialization in biomedical engineering. The present study sought to expand on existing research to understand factors that influence biomedical engineering students’ choice of intra-major specializations and how, if at all, these factors are related to gender. Grounded in social cognitive career theory, the present study leveraged quantitative surveys from undergraduate biomedical engineering students to understand factors influencing intra-major specialization choice, including the impact that students viewed on their career plans. Participants rated multiple factors as important in their intra-major specialization decisions, with professors/classes rated as the most important influence and alumni as the lowest. Similarly, participants rated multiple outcome expectations of their specialization, although income was rated lower than other factors. Participants most commonly indicated interest in pursuing careers in industry and medicine. We found some differences in intra-major specialization, outcome expectations, and career interests by gender, with women students indicating a higher influence of professors/classes and higher expectations for their track decision to provide a career with a good income. Further understanding of how undergraduate students select specializations in engineering coursework will inform curriculum design and student advising.